High diversity of lysis‐resistant cells upon the application of targeted physical and chemical lysis to environmental samples originating from three different water bodies

In most habitats, fluctuating environmental conditions create periods of compromised survival for metabolically active organisms. response, various strategies have evolved, including the formation resilient resting cells. We assessed biodiversity lysis-resistant bacteria in three different environments by applying a harsh physicochemical treatment to samples. The bacterial diversity fraction was compared with from same samples without application enrichment method. As expected, fraction, significantly higher relative abundance endospore-forming Firmicutes (for instance, Bacillus, Clostridium and Paenisporosarcina) observed comparison untreated However, genera which existence resistant cell form is not yet reported were also highly enriched Our results suggest more diversified repertoire cellular structures than previously thought.